High density DNA methylation array with single CpG site resolution
نویسندگان
چکیده
منابع مشابه
Global analysis of methylation profiles from high resolution CpG data.
New high throughput technologies are now enabling simultaneous epigenetic profiling of DNA methylation at hundreds of thousands of CpGs across the genome. A problem of considerable practical interest is identification of large scale, global changes in methylation that are associated with environmental variables, clinical outcomes, or other experimental conditions. However, there has been little...
متن کاملTitle: Estimating Absolute Methylation Levels at Single CpG Resolution from Methylation Enrichment and Restriction Enzyme Sequencing Methods Running title: methylCRF predicts single CpG resolution methylome
1 Department of Genetics, Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St. Louis, MO 63108 2 Department of Computer Science and Engineering, Washington University in St. Louis, St. Louis, MO 63130 3 Department of Dermatology, University of California San Francisco, CA 94143 4 Brain Tumor Research Center, Department of Neurosurgery, Helen Diller Famil...
متن کاملEstimating absolute methylation levels at single-CpG resolution from methylation enrichment and restriction enzyme sequencing methods
Estimating absolute methylation levels at single-CpG resolution from methylation enrichment and restriction enzyme sequencing methods Michael Stevens, Jeffrey B. Cheng, Daofeng Li, Mingchao Xie, Chibo Hong, Cécile L. Maire, Keith L. Ligon, Martin Hirst, Marco A. Marra, Joseph F. Costello, and Ting Wang Department of Genetics, Center for Genome Sciences and Systems Biology, Washington University...
متن کاملSystematic cross-validation of 454 sequencing and pyrosequencing for the exact quantification of DNA methylation patterns with single CpG resolution
BACKGROUND New high-throughput sequencing technologies promise a very sensitive and high-resolution analysis of DNA methylation patterns in quantitative terms. However, a detailed and comprehensive comparison with existing validated DNA methylation analysis methods is not yet available. Therefore, a systematic cross-validation of 454 sequencing and conventional pyrosequencing, both of which off...
متن کاملMethylation detection oligonucleotide microarray analysis: a high-resolution method for detection of CpG island methylation
Methylation of CpG islands associated with genes can affect the expression of the proximal gene, and methylation of non-associated CpG islands correlates to genomic instability. This epigenetic modification has been shown to be important in many pathologies, from development and disease to cancer. We report the development of a novel high-resolution microarray that detects the methylation statu...
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ژورنال
عنوان ژورنال: Genomics
سال: 2011
ISSN: 0888-7543
DOI: 10.1016/j.ygeno.2011.07.007